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KMID : 0380620210530060682
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Korean Journal of Food Science and Technology
2021 Volume.53 No. 6 p.682 ~ p.687
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Validation and measurement uncertainty of HPLC-UV method for quercetin quantification in various foods
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Seo Eun-Bin
Lim Su-Ji
Yun Choong-In
Shin Jae-Wook
Kim Young-Jun
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Abstract
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The purpose of this study was to validate a high-performance liquid chromatography (HPLC) method for the quantitative analysis of quercetin in various foods. The method was based on HPLC-UV (360 nm). The method was validated using candy, beverage, and sausage which were tested for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy, and the measurement uncertainty was assessed. Matrix-matched calibration was also applied. The calibration curves (0.5-50 mg/L) showed good linearity (r2≥0.9998). LOD and LOQ ranged from 0.15 to 0.31 mg/kg and from 0.44 to 0.93 mg/kg, respectively. The average accuracy and precision at 0.5, 2.5, and 10 mg/kg ranged from 84.3 to 102.0% and 0.7 to 3.0 relative standard deviation (RSD%), respectively. This study confirmed the applicability of the proposed method by applying it to commercial products, such as teas and beverages. Thus, the proposed analytical method is suitable for quantifying quercetin in various foods.
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KEYWORD
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quercetin, food additive, measurement uncertainty, validation
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